| Achievement | How to unlock |
| Baneful Payback (25 points) | Defeat Bane.
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| Born Free (10 points) | Escape from Intensive Treatment to the island surface.
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| Breaking And Entering (10 points) | Gain access to Arkham Mansion after it is locked down by the Joker.
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| Crocodile Tears (50 points) | Venture into Killer Croc's lair and come out alive.
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| Daydreamer (10 points) | Survive the nightmare of the Scarecrow's fear gas.
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| Double Trouble (25 points) | Defeat two Titan Henchmen at once.
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| Just What The Doctors Ordered (10 points) | Save all the doctors in Medical.
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| Leave No Man Behind (10 points) | Rescue the guards and henchman from the Joker toxin in Decontamination.
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| Malpractice Needs More Practice (10 points) | Survive the onslaught from the deformed Joker henchman.
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| Poisoned Ivy (50 points) | Defeat the giant Titan Ivy plant.
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| Recurring Nightmare (10 points) | Face your biggest fears and keep your sanity.
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| Resist The Fear (50 points) | Conquer the effects of the Scarecrow's fear gas.
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| Shocking Rescue (10 points) | Take down Zsasz in the Patient Pacification Chamber.
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| Solitary Confinement (25 points) | Capture and lock up Harley Quinn.
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| Zsasz Cut Down To Size (10 points) | Save Dr. Young from being killed by Victor Zsasz.
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