| CharacterHow to Unlock
|
| Hidden Mugenjo | Finish 100 Floors of Mugenjo
|
| Itachi | Finish 30 Floors of Hidden Mugenjo
|
| Itachi | Beat Heroes mode on Insane
|
| Jiraiya | Beat Heroes mode on Hard
|
| Jiraya | Finish 100 Floors of Mugenjo
|
| Kabuto | Finish 100 Floors of Mugenjo
|
| Kisame | Finish 30 Floors of Hidden Mugenjo
|
| Kisame | Beat Heroes mode on Insane
|
| Orochimaru | Finish 100 Floors of Mugenjo
|
| Orochimaru | Beat Heroes mode on Hard
|
| Sasuke | Finish 100 Floors of Mugenjo
|
| Shizune | Finish 100 Floors of Mugenjo
|
| Third Hokage | Finish 30 Floors of Hidden Mugenjo
|
| Tsunade | Finish 100 Floors of Mugenjo
|
| Tsunade | Beat Heroes mode on Hard
|
