Complete the indicated task in Story mode/Grand Prix to unlock the corresponding character signature card.
| Card | How to unlock |
| Bolt Tanner | Defeat Bolt Tanner in Stage 6-B.
|
| Burning Skull Head | Defeat Hunter Pace in Stage 6-A.
|
| Facility Chief's Duel Runner | Defeat Facility Chief in Stage 3.
|
| Greiger (Matchup Mode) | Defeat Greiger in Stage 7.
|
| Hoodlum's Duel Runner | Defeat Hoodlum in Stage 1.
|
| Hunter Pace (Matchup Mode) | Defeat Hunter Pace in Stage 6-A.
|
| Jack Atlas (Matchup Mode) | Complete Story Mode twice.
|
| Leo's Duel Runner | Defeat Leo in Stage 2-A.
|
| Luna's Duel Runner | Defeat Luna in Stage 5-B.
|
| Mad Archfiend | Complete Story mode twice.
|
| Normal Duel Runner(Bolt Tanner's Duel Runner) | Defeat Bolt Tanner in Stage 6-B.
|
| Officer Tetsu Trudge (Matchup Mode) | Defeat Officer Tetsu Trudge in Stage 4.
|
| Officer Tetsu Trudge's Duel Runner | Defeat Officer Tetsu Trudge in Stage 4.
|
| Security 2's Duel Runner | Win the Silver Cup in first place.
|
| Skull Collector | Defeat Hoodlum in Stage 1-A.
|
| Sparks | Defeat Greiger in Stage 7 two times.
|
| Stardust Dragon | Defeat Story Mode once.
|
| Summon Reactor-SK | Defeat Greiger in Stage 7.
|
| Sunlight Unicorn | Defeat Luna in Stage 5-B.
|
| Tremendous Fire | Defeat Hunter Pace in Stage 6-A two times.
|
| Ushi Oni | Defeat Bolt Tanner in Stage 6-B.
|
| Wall of Ivy | Defeat Akiza in Stage 5-A.
|
| Wheel of Fortune (Jack Atlas' Duel Runner) | Complete Story mode twice.
|
| Yusei Fudo (Matchup Mode) | Complete Story mode.
|
| Yusei's Duel Runner | Complete Story mode.
|
